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UV-VIS analysis, short for Ultraviolet-Visible Spectroscopy, is a widely used analytical technique that involves measuring the absorption of ultraviolet and visible light by a sample. This method provides valuable information about the chemical composition, concentration, and structural properties of molecules in a sample.
In UV-VIS spectroscopy, a beam of UV and/or visible light is passed through the sample, and the amount of light absorbed by the sample at different wavelengths is measured. The absorption spectrum obtained reveals the absorption bands or peaks characteristic of the sample's components.
UV-VIS analysis is commonly used in various fields, including pharmaceuticals, environmental science, food and beverage, materials science, and biochemistry. Some typical applications include:
Quantitative analysis: UV-VIS spectroscopy is often used for quantitative analysis of compounds with known absorption spectra. By measuring the absorbance of a sample at a specific wavelength and comparing it to a calibration curve, the concentration of the analyte can be determined. Quantitative determination of transition metal ions, highly conjugated organic compounds, and biological macromolecules.
Qualitative analysis: UV-VIS spectroscopy can also be used for qualitative analysis, where the presence or absence of certain compounds in a sample is identified based on their characteristic absorption spectra.
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